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Install NowCBSE Syllabus of Class 12 Biotechnology – in PDF
CBSE Syllabus for Class 12 Biotechnology 2019-20 is now available in myCBSEguide mobile app. contains all the topics of this session. myCBSEguide provides you latest Syllabus for Class 12 Biotechnology in PDF format for free download. Biotechnology is a broad discipline in which biological processes, organisms, cells or cellular components are exploited to develop new technologies. The curriculum for March 2020 exams is designed by CBSE, New Delhi as per NCERT textbooks for the session 2019-20.
CBSE Syllabus for class 12 Biotechnology 2019-20
CBSE Syllabus Class 12 Biotechnology
COURSE- STRUCTURE-(THEORY)
One Paper
Max. Marks 70+30
Time: 3 hrs.
| Units | No. of Periods | Marks | |
|---|---|---|---|
| Unit V | Protein and Gene Manipulation | 100 | 40 |
| Unit VI | Cell Culture and Genetic Manipulation | 80 | 30 |
| Practicals | 60 | 30 | |
| Total | 240 | 100 |
One Paper
Total Marks: 70
Time: 3 hrs.
Unit-V Protein and Gene Manipulation (40 Marks)
Chapter-1: Recombinant DNA Technology
Introduction, Tool of DNA technology, Making DNA, Introduction of recombinant DNA into host cells, Identification of Recombinants, Polymerase Chain Reaction (PCR), Hybridization Techniques, DNA Library, DNA Sequencing, Site-directed Mutagenesis
Chapter-2: Protein Structure and Engineering
Introduction to the world of proteins, 3-D shape of proteins, Structure-Function Relationship in Proteins, Purification of Proteins, Characterization of Proteins, Protein-Based Products, Designing Proteins (Protein Engineering)
Chapter-3: Genomics, Proteomics, and Bioinformatics
Introduction, Genome, Sequencing Projects, Gene prediction and counting, Genome Similarity, SNPs and Comparative Genomics, Functional Genomics, Proteomics, History of Bioinformatics, Sequences and nomenclature, Information Sources, Analysis using Bioinformatics tools
Unit-VI Cell Culture and Genetic Manipulation (30 Marks)
Chapter-1: Microbial Cell Culture and its Applications
Introduction, Microbial Nutrition, and Culture Techniques, Measurement and Kinetics of Microbial Growth, Scale-up of Microbial Process, Isolation of Microbial Products, Strain Isolation and Improvement, Applications of Microbial Culture Technology, Biosafety Issues in Microbial Technology
Chapter -2: Plant Cell Culture and Applications
Introduction, Cell and Tissue Culture Techniques, Applications of Cell and Tissue Culture, Gene Transfer Methods in Plants, Transgenic Plants with Beneficial Traits, Biosafety of Transgenic Plants
Chapter-3: Animal Cell Culture and Applications
Introduction, Animal Cell Culture Techniques, Characterization of Cell lines, Methods of Gene Delivery into Cells, Scale-up of Animal Culture Process, Applications of Animal Cell Culture, Stem Cell Technology, Tissue Engineering
PRACTICALS (30 Marks)
Note: Every student will be required to do the following experiments during the academic session.
- Use of special equipment in biotechnology experiments
- Isolation of bacterial plasmid DNA
- Detection of DNA by gel electrophoresis
- Isolation of Genomic DNA (CTAB method)
- Estimation of DNA
- Bacterial transformation using any plasmid
- Restriction digestion of plasmid DNA & its analysis by gel electrophoresis
- Isolation of bacteria from curd & staining of bacteria
- Cell viability assay
- Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it
- Reading of a DNA sequencing gel to arrive at the sequence
Project work
Scheme of Evaluation
Time: 3 Hours
Max. Marks 30
The scheme of evaluation at the end of the session will be as under:
| A | Two experiments | 6+6 (only one computer-based practical) |
| Practical record | 04 | |
| Viva on Practical | 04 | |
| B | Project work | |
| Write up | 05 | |
| Viva on project | 05 | |
| Total | 30 |
Prescribed Books:
- A Text Book of Biotechnology – Class XII: Published by CBSE, New Delhi
- A Laboratory Manual of Biotechnology – Class XII: Published by CBSE, New Delhi
Question Paper Design
1) Board Examination – Theory
Time: 3 Hours
Max. Marks: 70
| S.No. | Typology of Questions | VSA (1 Marks) | SA-I (2 Marks) | SA-II (3 marks) | LA (5 marks) | Total Marks | Percent -age |
|---|---|---|---|---|---|---|---|
| 1. | Remembering- Exhibit memory of previously learned material by recalling facts, terms, basic concepts and answers. | 5 | 1 | 2 | 1 | 9 (18) | 25.7% |
| 2. | Understanding- Demonstrate understanding of facts and ideas by organizing, comparing, translating, interpreting, giving descriptions and stating main ideas. | 4 | 1 | 2 | 1 | 8 (17) | 24.3% |
| 3. | Applying: Solve problems to new situations by applying acquired knowledge, facts, techniques and rules in a different way. | 3 | 1 | 1 | 1 | 6 (13) | 18.5% |
| 4. | Analyzing & Evaluating: Examine and break information into parts by identifying motives or causes. Make inferences and evidence to support generalizations Present and defend opinions by making judgment about information, validity of ideas or quality of work based on a set of criteria. | 2 | 2 | 1 | 1 | 6 (14) | 20% |
| 5. | Creating: Compile information together in a different way by combining elements in a new pattern or proposing alternative solutions | 1 | 2 | 1 | – | 4 (8) | 11.5% |
| TOTAL | 15X1=15 | 7X2=14 | 7X3=21 | 4X5=15 | 70 |
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