What are shuttle vector
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Posted by Sumit Gour 3 years, 11 months ago
- 3 answers
Gaurav Seth 3 years, 11 months ago
A shuttle vector is defined as a plasmid capable of replicating in two different organisms.
Vectors are the DNA molecules that carry a foreign DNA segment and replicate within the host cell such as bacteriophages, cosmids, phagemid, BAC (bacterial artificial chromosomes), YAC (yeast artificial chromosome) animal and plant vectors, and shuttle vectors.
Plasmid DNA acts as a vector to transfer a piece of alien DNA attached to it.
Recombinant DNA = vector + insert
Mosquito is an insect vector that transfers the malarial parasite into the human body, similarly, the plasmid is used to transfer alien DNA into the host organism.
Shuttle vectors are those vectors that can be used for more than one organism like used for both prokaryotes and eukaryotes.
Yogita Ingle 3 years, 11 months ago
A shuttle vector is a vector constructed so that it can propagate in two different host species. Therefore, DNA inserted into a shuttle vector can be tested or manipulated in two different cell types. The main advantage of these vectors is they can be manipulated in E. coli, then used in a system which is more difficult or slower to use (e.g. yeast).
Shuttle vectors include plasmids that can propagate in eukaryotes and prokaryotes or in different species of bacteria . There are also adenovirus shuttle vectors, which can propagate in E. coli and mammals.
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Anushka Singh 3 years, 9 months ago
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