Why are ddNTPs used in sequencing? …
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Meghna Thapar 5 years, 1 month ago
DdNTP are useful in the analysis of DNA's structure as it stops the polymerisation of a DNA strand during a DNA replication, producing different lengths of DNA strands replicated from a template strand. Chain termination sequencing involves the synthesis of new strands of DNA complementary to a single-stranded template (step I). The template DNA is supplied with a mixture of all four deoxynucleotides, four dideoxynucleotides--each labeled with a different color fluorescent tag, and DNA polymerase (step II).
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