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Why it is useful to search a database to identity newly determined DNA sequence

    • Posted by Sheetal Damara 5 years, 10 months ago

    CBSE > Class 12 > Biotechnology
    • 1 answers

    Meghna Thapar 4 years, 3 months ago

    DNA sequencing is the process used to determine the order of nucleotides in a specific DNA molecule. This information is useful for researchers in understanding the type of genetic information that is carried in the DNA, which may affect its function in the body. A computer program can be used to check an unknown DNA sequence for ORFs. The program transcribes each DNA strand into its complementary RNA sequence and then translates the RNA sequence into an amino acid sequence. Each DNA strand can be read in three different reading frames.

    Steps in DNA sequencing:

    1. Sample preparation (DNA extraction)
    2. PCR amplification of target sequence.
    3. Amplicons purification.
    4. Sequencing pre-prep.
    5. DNA Sequencing.
    6. Data analysis.

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