What is acid soluble pool and …
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Meghna Thapar 5 years, 2 months ago
The acid insoluble pool contains some macromolecular organic compounds like nucleic acids, proteins, carbohydrates. Exceptionally lipid which is a micromolecular compound is also found in the acid insoluble fraction as it forms globules. Other micromolecular compounds are found in the acid soluble fraction.
When a living tissue is ground in trichloroacetic acid, a thick slurry is formed as a result. Upon filtration of this slurry. We will get two fractions, the filtrate called as the acid soluble pool and the retentate known as the acid insoluble pool.
Both micromolecules and macromolecules can be found in both of the soluble and insoluble pools.
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