Chemiosmotic hypothesis mein ATP bante hai …

As shown in the above diagram it can be stated that ATP production in case of photoionization of chlorophyll follow the chemiosmotic hypothesis of ATP synthesis

According to the Chemiosmotic hypothesis the ATP is synthesized in respiring cells from the electrochemical gradiance across the inner membrane of the mitochondria by using the energy of NADH and FADH₂ formed from the breaking down of the energy rich molecules such as glucose.

 Photoactivation of chlorophyll a results in the splitting of water molecules and the transfer of energy to ATP and reduced nicotinamide adenine dinucleotide phosphate (NADP).

• light energy is trapped by chlorophyll to make ATP (photophosphorylation)

• at the same time water is split into oxygen, hydrogen ions and free electrons:

  2H₂O    4H⁺ + O₂ + 4e^- (photolysis)

• the electrons then react with a carrier molecule nicotinamide adenine dinucleotide phosphate (NADP), changing it from its oxidised state (NADP⁺) to its reduced state (NADPH):

  NADP⁺ + 2e^- + 2H⁺    NADPH + H⁺ 

 The components of non-cyclic phosphorylation are found in the thylakoid membranes of the chloroplast. Electrons passing through the transport chain provide energy to pump H⁺ ions from the stroma, across the thylakoid membrane into the thylakoid compartment. H⁺ ions are more concentrated in the thylakoid compartment than in the stroma. We say there is an electrochemical gradient. H⁺ ions diffuse from the high to the low regions of concentration. This drives the production of ATP.