Plz explain dna fingerprinting method

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Amar Kumar 8 years, 3 months ago
DNA fingerprinting is a technique that simultaneously detects lots of minisatellites in the genome to produce a pattern unique to an individual. This is a DNA fingerprint.
The probability of having two people with the same DNA fingerprint that are not identical twins is very small.
Just like your actual fingerprint, your DNA fingerprint is something you are born with, it is unique to you.
RFLP(Restriction fragment length polymorphism) :RFLP analyzes the length of the strands of the DNA molecules with repeating base pair patterns. DNA molecules are long strands found tightly wound in chromosomes which are contained in the nucleus of each human cell. Within each DNA strand are numbers of genes that determine the particular characteristics of an individual. While about 5% of the gene compositions on DNA contain this type of genetic information, the other 95% do not. However, of the 95%, these non-coding genes contain identifiable repetitive sequences of base pairs, which are called Variable Number Tandem Repeats (VNTR). To extract a DNA fingerprint, a Southern blot is performed and the DNA is analyzed via a radioactive probe. The restriction fragment length polymorphism analysis is used to detect the repeated sequences by determining a specific pattern to the VNTR, which becomes the person's DNA fingerprint.
The drawback with this system is that it requires a considerable amount of DNA in order to be used.
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