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Why annealing is Important in PCR?

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Why annealing is Important in PCR?

    • Posted by Aradhna Vidyarthi 4 years, 11 months ago

    CBSE > Class 12 > Biology
    • 1 answers

    Tanya ?? 4 years, 11 months ago

    During the annealing phase of PCR, the reaction temperature needs to be sufficiently low to allow both forward and reverse primers to bind to the template, but not so low as to enable the formation of undesired, non-specific duplexes or intramolecular hairpins, both of which reduce reaction efficiency.

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