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Why can't DNA polymerase be used …

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Why can't DNA polymerase be used in PCR
  • 2 answers

Devil ? 4 years, 11 months ago

Degenerate at high temperature

Yogita Ingle 4 years, 11 months ago

T.aquaticus or Thermus aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions like high temperature required during PCR. Therefore is the source of the DNA polymerase used in PCR technique.

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