The same restriction endonuclease cuts both …
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Meghna Thapar 5 years ago
Restriction enzymes cut DNA bonds between 3′ OH of one nucleotide and 5′ phosphate of the next one at the specific restriction site. Adding methyl groups to certain bases at the recognition sites on the bacterial DNA blocks the restriction enzyme to bind and protects the bacterial DNA from being cut by themselves. Each restriction enzyme cuts DNA at a different restriction site. Some restriction enzymes make staggered cuts through a DNA molecule, producing. Restriction enzymes cut through both nucleotide strands, breaking the DNA into fragments, but they don't always do this in the same way. This overhanging nucleotide strand is called a sticky end because it can easily bond with complementary DNA fragments.
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