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Gaurav Seth 5 years, 1 month ago
The steps in the production of human insulin by genetic engineering method includes:
1. Human insulin is extracted from pancreas cells and an insulin-producing gene is isolated.
2. A plasmid DNA is extracted from a bacterium and cut with restriction enzyme, forming plasmid vector.
3. Insert human insulin-producing gene into the bacterial plasmid vector to form the recombinant DNA of human insulin-producing gene.
4. Introduce this recombinant DNA into a bacterial cell to form the recombinant bacterium.
5. The recombinant bacteria multiply in a fermentation tank and produce human insulin.
6. Insulin is extracted, purified and bottled. It is then ready to be injected into diabetic patients.
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