Explain the over all steps involved …
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Meghna Thapar 5 years, 2 months ago
The first step in rDNA technology is to isolate the desired DNA in its pure form i.e. free from other macromolecules. ... Other macromolecules are removable with other enzymes or treatments. Ultimately, the addition of ethanol causes the DNA to precipitate out as fine threads. This is then spooled out to give purified DNA. Recombinant DNA technology. This technology has five steps: (1) cutting the desired DNA by restriction sites, (2) amplifying the gene copies by PCR, (3) inserting the genes into the vectors, (4) transferring the vectors into host organism, and (5) obtaining the products of recombinant genes.
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