Explain the machanism of transcription in …

Transcription in prokaryotes:

• Initiation: In this step RNA polymerase enzyme along with initiation factor (sigma) binds with DNA sequence at promotor and starts transcription.
• Elongation: In this step RNA polymerase enzyme nucleoside triphosphate act as a substrates and polymerises the nucleotides of templates as a complementary strand.
• Termination: In this step terminator factor (rho) replaces the initiation factor at the termination point of DNA sequence.At this stage detachment of RNA and RNA polymerase enzyme with rho factor takes place.

In eukaryotes, transcription occrurs in nucleus whereas in prokaryotes it occurs in cytoplasm.
In eukaryotes RNA processing is required as its structural gene is monocistronic whereas in prokaryotes it does not require because its structural gene is polycistronic.