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What is the process of annealing …

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What is the process of annealing in PCR in chapter 11 dna recombination
  • 2 answers

Kriti Sharma 5 years, 6 months ago

PCR.................. polymerase Chain Reaction................. basically in this we just want to create many DNA copies ok................ so first step that we are doing is ..................denaturation.......... that is we are separating both the strands of DNA by the application of high temperature.................. now we cannot just put all the primers in one go................. and make new DNA .................primers are added slowly in small small amount ...........that process is called annealing.................. then they become a long chain of nucleotides...................... DNA is not prepared in one go we need to put some primers Anneal them .....................and they will undergo polymerization to form complete dna strand...........???

Yogita Ingle 5 years, 6 months ago

  • Annealing is the process of pairing of primers with complimentary base sequences of the two separated strands.
  • Primers are single strand sequences of DNA or RNA around 20 to 30 bases in length.
  • They serve as the starting point for the synthesis of DNA.
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