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4. A biotechnologist wanted to create …

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4. A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?
  • 2 answers

Aleena Elizabeth Vinod 6 years, 1 month ago

Sal l

Yogita Ingle 6 years, 1 month ago

The biotechnologist can ligate the foreign DNA at the Sal I of Tetracycline resistance gene in pBR322 vector.

 

• Since the Tetracycline resistance gene has 2 restriction sites, namely BamH I and Sal I, the foreign DNA can be ligated at any of these 2 sites.

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