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Yogita Ingle 6 years, 2 months ago
(a) Restriction Endonucleases These are the bacterial enzymes that cut dsDNA into fragments after recognising and binding to the specific nucleotide sequences, known as recognition site. These enzymes are used to form recombinant molecules of DNA, composed of DNA from different sources.
(b) Gel-Electrophoresis is the technique which allows the visualisation of separated fragments of DNA on an agarose gel matrix.
Since, the DNA fragments are negatively charged molecules, they separate and move towards the anode (+ ve) under the influence of an electric field. DNA fragments are separated on the basis of their size through the sieving effect provided by the gel.
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