How do restriction endonucleases cut DNA?
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Posted by Nandini Gupta 6 years, 2 months ago
- 2 answers
Yogita Ingle 6 years, 2 months ago
- Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands which leaves a single stranded portions at the ends and the overhanging stretches called sticky ends on each strand.
- When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together using DNA ligases.
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Aa Hh 6 years, 2 months ago
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