To use the restriction enzymes for …

S ince the DNA is enclosed within the m embranes, we have to break the cell open to release DNA along with othe r macromolecules such as RNA, proteins, polysaccharides and also lipids . This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). DNA that separates out can be removed by spooling You know that genes are located on long molecules of DNA interwined with proteins such as histones. The RNA can b e removed by treatment with ribonuclease whereas proteins can b e removed by treatment with proteas e. Other molecules can be removed by appropriate treatments and purified DNA ultimately precipitates out after the addition of chilled ethanol. This can be seen as collection of fine threads in the suspension