Is the restriction endonuclease found in …
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Meghna Thapar 5 years, 8 months ago
Restriction enzymes are like scissors; they cut up DNA at specific regions. These enzymes are found in bacteria (prokaryotes) but not in eukaryotic cells. Naturally occurring restriction endonucleases are categorized into four groups (Types I, II, III, and IV) based on their composition and enzyme cofactor requirements, the nature of their target sequence, and the position of their DNA cleavage site relative to the target sequence. No, eukaryotic cells do not have restriction endonucleases. This is because the DNA of eukaryotes is highly methylated by a modification enzyme, called methylase. Methylation protects the DNA from the activity of restriction enzymes. These enzymes are present in prokaryotic cells where they help prevent the invasion of DNA by a virus.
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