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Describe various steps of gene cloning.

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Describe various steps of gene cloning.

    • Posted by Nozrul Islam 6 years, 5 months ago

    CBSE > Class 12 > Biology
    • 2 answers

    Sia ? 6 years, 5 months ago

    Steps involved in gene cloning

    1. Isolation of donor DNA fragment or gene
    2. Selection of suitable vector
    3. Incorporation of donor DNA fragment into the vector
    4. Transformation of recombinant vector into a suitable host cell
    5. Isolation of recombinant host cell

    1Thank You

    Gursharn Kaur 6 years, 5 months ago

    *Isolation of a genetic material is DNA *Cutting of DNA at specific locations formation of Recombinant DNA *Amplification of gene of interest using PCR *Preparation and insertion of Recombinant DNA into host cell organism * obtaining the foreign gene product * downstream processing

    1Thank You

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