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What is insertional inactivation ?????????

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What is insertional inactivation ?????????
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Shagun Waraich 7 years, 9 months ago

In insertional activation a recombinant DNA is inserted with the coding sequence of beta-glycosidase. which result into inactivation of enzyme. The bacterial colonies have inserted plasmid do not show coloration while those without plasmid show blue colour in the presence of chromogenic subtrate
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