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| Prokaryotic Replication | Eukaryotic Replication |
| This process occurs in prokaryotic cells. | This process occurs in eukaryotic cells. |
| It is a continuous process. | This process occurs in the S-phase of cell cycle. |
| Circular, double-stranded DNA | Linear, double-stranded DNA with end |
| The DNA replicates in the cytoplasm | The DNA replicates in the nucleus |
| Single origin of replication | Multiple origins of replication |
| Small amount of DNA | The DNA is 50 times more than prokaryotic DNA |
| DNA polymerase I and III are involved | DNA polymerase ɑ, δ and ε are involved. |
| Large okazaki fragments | Small okazaki fragments |
| The process is rapid, 2000 base pairs per second | The process is slow, 100 base pairs per second |
| Two circular chromosomes are obtained | Two sister chromatids are obtained |
| DNA gyrase is required | DNA gyrase is not required |
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