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Yogita Ingle 7 years, 5 months ago
There are three major types of RNAs in bacteria:
- Messenger RNA (mRNA) – It provides the template to make protein.
- Transfer RNA (tRNA) – It reads the genetic code and transfers amino acids for protein synthesis.
- Ribosomal RNA (rRNA) – It has a structural and catalytic role in protein synthesis.
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Yogita Ingle 7 years, 5 months ago
DNA fingerprinting is the technique of finding the difference between the satellite DNA regions in the genome. These regions are stretches of repetitive DNA which do not code for any specific protein. These non-coding sequences form a major chunk of the DNA profile of humans. They depict a high level of polymorphism and are the basis of DNA fingerprinting. These genes which show a high level of polymorphism in all kind of tissues as a result of which they prove to be very useful in forensic studies.
Any piece of DNA sample found at a crime scene can be analysed for the level of polymorphism in the non-coding repetitive sequences. After the DNA profile is traced, it becomes easier to find the criminal by performing the DNA fingerprinting for the suspects. Apart from crime scenes, Fingerprinting applications also prove useful in finding the parents of an unclaimed baby by conducting Paternity test on a DNA sample from the baby.
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Yogita Ingle 7 years, 4 months ago
The tryptophan operon is the regulation of transcription of the gene responsible for biosynthesis of tryptophan from chorismate.
Tryptophan operon consists of structural gene and regulatory gene.
I. Regulatory gene are Promoter, Repressor, Operater and Leader sequence.
II. Structural gene are TrpE, TrpD, TrpC, TrpB and TrpA
- trpE: It enodes the enzyme Anthranilate synthase I
- trpD: It encodes the enzyme Anthranilate synthase II
- trpC: It encodes the enzyme N-5’-Phosphoribosyl anthranilate isomerase and Indole-3-glycerolphosphate synthase
- trpB: It encodes the enzyme tryptophan synthase-B sub unit
- trpA: It encode the enzyme tryptophan synthase-A sub unit
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