eukaryotic cells are preferred for expression …
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Meghna Thapar 3 years, 9 months ago
Gene expression in eukaryotic cells is regulated by repressors as well as by transcriptional activators. Like their prokaryotic counterparts, eukaryotic repressors bind to specific DNA sequences and inhibit transcription. ... Other repressors compete with activators for binding to specific regulatory sequences. Because host chromosomal genes are not synthesized at this point, these products are the only proteins synthesized. ... The T7 RNA polymerase then transcribes this gene at high levels, resulting in high production of the factor VIII protein in the bacteria.
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