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Why are ddNTP used in dna …

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Why are ddNTP used in dna sequencing

    • Posted by Shu Ku 5 years ago

    CBSE > Class 12 > Biotechnology
    • 2 answers

    Ishana Milli 4 years, 9 months ago

    Ddntp stands for dideoxyribose triphosphate. These molecules do not have oxygen on the 3rd and 2nd carbon positions on the bases. Thus they do not have free sites to react and make a bond unlike those sugars which have functional gruops attached on some positions to carbon providing free site to react and make a bond like deoxyribose and ribose sugar . By adding ddntps chain elongation can be inhibited because they result in the termination of the newly synthesised dna chains.

    1Thank You

    Ritu Kahar 5 years ago

    ddNTP are chain elongating inhibitors which can lead to the termination of the DNA sequence

    1Thank You

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