Explain with suitable diagram ,the principle …
CBSE, JEE, NEET, CUET
Question Bank, Mock Tests, Exam Papers
NCERT Solutions, Sample Papers, Notes, Videos
Related Questions
Posted by Tarani Balaji 5 months, 1 week ago
- 1 answers
Posted by Lubhi Sahu 2 years, 11 months ago
- 0 answers
Posted by Neha Araj 2 years, 7 months ago
- 0 answers
Posted by Pankaj Singh 1 year, 8 months ago
- 1 answers
Posted by ? Royal Thakur ? 2 years, 9 months ago
- 2 answers
myCBSEguide
Trusted by 1 Crore+ Students
Test Generator
Create papers online. It's FREE.
CUET Mock Tests
75,000+ questions to practice only on myCBSEguide app
Meghna Thapar 3 years, 9 months ago
Sanger Sequencing Steps
The Sanger sequencing method consists of 6 steps:
(1) The double-stranded DNA (dsDNA) is denatured into two single-stranded DNA (ssDNA).
(2) A primer that corresponds to one end of the sequence is attached.
(3) Four polymerase solutions with four types of dNTPs but only one type of ddNTP are added.
(4) The DNA synthesis reaction initiates and the chain extends until a termination nucleotide is randomly incorporated.
(5) The resulting DNA fragments are denatured into ssDNA.
(6) The denatured fragments are separated by gel electrophoresis and the sequence is determined.
0Thank You