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Differentiate between adherent ans suspension culture

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Differentiate between adherent ans suspension culture

Posted by Shreya Awasthi 5 years, 4 months ago

CBSE > Class 12 > Biotechnology

1 answers

Yogita Ingle 5 years, 4 months ago

<th>Adherent Cell Culture</th> <th>Suspension Cell Culture</th>

Appropriate for most cell types, including primary cultures	Appropriate for cells adapted to suspension culture and a few other cell lines that are nonadhesive (e.g., hematopoietic)
Requires periodic passaging, but allows easy visual inspection under inverted microscope	Easier to passage, but requires daily cell counts and viability determination to follow growth patterns; culture can be diluted to stimulate growth
Cells are dissociated enzymatically or mechanically	Does not require enzymatic or mechanical dissociation
Growth is limited by surface area, which may limit product yields	Growth is limited by concentration of cells in the medium, which allows easy scale-up
Requires tissue-culture treated vessel	Can be maintained in culture vessels that are not tissue-culture treated, but requires agitation (i.e., shaking or stirring) for adequate gas exchange
Used for cytology, harvesting products continuously, and many research applications	Used for bulk protein production, batch harvesting, and many research applications

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