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Download CBSE class 12 Biotechnology study material in PDF format. MyCBSEguide provides solved papers, board question papers, revision notes and NCERT solutions for CBSE class 12 Biotechnology. The topics included are antibiotics, vaccines, monoclonal antibodies and many more. Furthermore, developments in recombination DNA technology have yielded numerous new useful products in the fields of healthcare and agriculture.

Unit-V Protein and Gene Manipulation (40 Marks)

Chapter-1: Recombinant DNA Technology Introduction, Tool of rDNA technology, Making rDNA, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase chain reaction (PCR), Hybridization techniques, DNA library, DNA sequencing, Site-directed mutagenesis

Chapter-2: Protein Structure and Engineering Introduction to the world of proteins, 3-D shape of proteins, Structure-function relationship in proteins, Purification of proteins, Characterization of proteins, Protein based products, Designing proteins (protein engineering)

Chapter-3: Genomics and Bioinformatics Introduction, Genome sequencing projects, Gene prediction and counting, Genome similarity, SNPs and comparative genomics, Functional genomics, Proteomics, History of bioinformatics, Sequences and nomenclature, Information sources, Analysis using bioinformatics tools

Unit-VI Cell Culture and Genetic Manipulation (30 Marks)

Chapter-1: Microbial Culture and Applications

Introduction, Microbial culture techniques, Measurement and kinetics of microbial growth, Scale up of microbial process, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology, Biosafety issues in microbial technology

Chapter-2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Gene transfer methods in plants, Transgenic plants with beneficial traits, Biosafety in plant genetic engineering

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Characterisation of cell lines, Methods of gene delivery into cells, Scale-up of animal culture process, Applications of animal cell culture, Stem cell technology, Tissue engineering

PRACTICALS (30 Marks)

Note: Every student will be required to do the following experiments during the academic session.

List of Experiments
1. Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
2. Bacterial transformation using any plasmid
3. Sterilization techniques
4. Preparation of bacterial growth medium
5. Isolation of bacteria from curd and staining of bacteria
6. Determination of bacterial growth curve
7. Cell viability assay
8. Data retreival and data base search using internet site NCBI
9. Download a DNA and protein sequence from internet, analyse it and comment on it
10. Reading of DNA sequencing gel to arrive at the sequence
11. Project work

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