{"id":23369,"date":"2018-12-11T10:25:00","date_gmt":"2018-12-11T04:55:00","guid":{"rendered":"http:\/\/mycbseguide.com\/blog\/?p=23369"},"modified":"2018-12-17T11:05:39","modified_gmt":"2018-12-17T05:35:39","slug":"cbse-question-paper-2007-class-12-biotechnology","status":"publish","type":"post","link":"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/","title":{"rendered":"CBSE Question Paper 2007 class 12 Biotechnology"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_76 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 eztoc-toggle-hide-by-default' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/#Last_Year_Question_Paper_Class_12_Biotechnology_2007\" >Last Year Question Paper Class 12 Biotechnology 2007<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/#SECTION_A\" >SECTION A<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/#SECTION_B\" >SECTION B<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/#SECTION_C\" >SECTION C<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/#SECTION_D\" >SECTION D<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/#CBSE_Question_Paper_2007_class_12_Biotechnology\" >CBSE Question Paper 2007 class 12 Biotechnology<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/#Previous_Year_Question_Paper_for_class_12_in_PDF\" >Previous Year Question Paper for class 12 in PDF<\/a><\/li><\/ul><\/nav><\/div>\n<p><strong>CBSE Question Paper 2007 class 12 Biotechnology<\/strong>\u00a0conducted by Central Board of Secondary Education, New Delhi in the month of March 2007. CBSE previous year question papers with solution are available in myCBSEguide mobile app and cbse guide website. The Best CBSE App for students and teachers is myCBSEguide which provides complete study material and practice papers to cbse schools in India and abroad.<\/p>\n<p style=\"text-align: center;\"><strong>CBSE Question Paper 2007 class 12 Biotechnology<\/strong><\/p>\n<p style=\"text-align: center;\"><strong><a class=\"button\" href=\"https:\/\/mycbseguide.com\/downloads\/cbse-class-12-biotechnology\/1849\/cbse-last-year-papers\/3\/\">Download as PDF<\/a><\/strong><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Last_Year_Question_Paper_Class_12_Biotechnology_2007\"><\/span>Last Year Question Paper Class 12 Biotechnology 2007<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<div>\n<div>\n<p><strong>General Instructions:<\/strong><\/p>\n<p>(i) All questions are compulsory.<br \/>\n(ii) There is no overall choice. However, an internal choice has been provided in one question of two marks and two questions of five marks. You have to attempt only one of the choices in such questions. Question paper contains four sections -A, B, C and D.<br \/>\n(iii) Question numbers 1 to 5 are very short answer questions, carrying 1 mark each.<br \/>\n(iv) Question numbers 6 to 15 are short answer questions, carrying 2 marks each.<br \/>\n(v) Question numbers 16 to 25 are also short answer questions, carrying 3 marks each.<br \/>\n(vi) Question numbers 26 to 28 are long answer questions, carrying 5 marks each.<br \/>\n(vii) Use of calculators is not permitted. However, you may use log tables, if necessary<\/p>\n<hr \/>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"SECTION_A\"><\/span><strong>SECTION A<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>1. Give the sequence of the two primers (5 nucleotides long) required to amplify the<br \/>\nfollowing DNA sequence by PCR: 1<br \/>\n5&#8242; GCACCTAGATCGATCC 3\u2019<\/p>\n<p>2. What is lyophilization?<\/p>\n<p>3. What treatment will you recommend to a fruit-seller for ripening a consignment of<br \/>\n\u2019Flavr Savr\u2019 tomatoes, and why?<\/p>\n<p>4. A soil micro-organism produces a novel metabolite in nanomolar concentration (nM).<br \/>\nSuggest a way to increase its production in quantities that are economically viable.<\/p>\n<p>5. Suppose you are planning a large scale hybridisation programme in maize, how can this task be made less labour intensive?<\/p>\n<hr \/>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"SECTION_B\"><\/span><strong> SECTION B<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>6. You wish to introduce the human insulin gene into a bacterial host in the hope of<br \/>\nproducing large amounts of human insulin. Should you use genomic DNA or cDNA?<br \/>\nExplain.<\/p>\n<p>7. What are ESTs? How are they useful in genome analysis?<\/p>\n<p>8. What is the mode of action of tissue plasminogen activator (t-PA)? Suggest one medical application of t-PA.<\/p>\n<p>9. Which of the following proteins would be expected to migrate fastest through SDS-PAGE gel, and why?<\/p>\n<table class=\"mobile\" border=\"1\" cellspacing=\"0\" cellpadding=\"3\">\n<tbody>\n<tr>\n<td style=\"width: 89px;\">Protein<\/td>\n<td style=\"width: 88px;\">MW (daltons)<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 89px;\">Transferrin<\/td>\n<td style=\"width: 88px;\">90,000<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 89px;\">Cytochrome c<\/td>\n<td style=\"width: 88px;\">13,400<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 89px;\"><em>\u03b1<\/em>\u03b1 -antitrypsin<\/td>\n<td style=\"width: 88px;\">45,000<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 89px;\">Myoglobin<\/td>\n<td style=\"width: 88px;\">17,000<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 89px;\">Serum albumin<\/td>\n<td style=\"width: 88px;\">69,000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>10. <\/strong>Give two distinguishing features of pBR322 and pUC19 vectors.<\/p>\n<p>11. What is the IUPAC code for A or C? Write the complementary sequence of the following sequence :<br \/>\n5&#8242;-ASGYTWCAG-3&#8242;<\/p>\n<p>12. Why is aeration important for microbial growth? How can proper aeration be achieved in microbial cultures grown under laboratory conditions?<\/p>\n<p>13. The long distance runners are disqualified if they test positive for erythropoietin (EPO). What is this substance and how does it act?<\/p>\n<p style=\"text-align: center;\"><strong>OR<\/strong><\/p>\n<p>Embryonic cells during development not only commit along different lineages but also<br \/>\nretain a population of cells that are present only at strategic locations in the adult<br \/>\norganism. What are these specialized cells known as? Why are they maintained in<br \/>\nundifferentiated state?<\/p>\n<p>14. An autoradiogram of a sequencing gel containing 4 lanes of DNA fragments is shown in the figure below:<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" src=\"http:\/\/media-mycbseguide.s3.amazonaws.com\/images\/cbse\/12\/biotechnology\/lyp\/img01.PNG\" alt=\"CBSE Question Paper 2007 class 12 Biotechnology\" width=\"189\" height=\"135\" \/><\/p>\n<p>(a) Read the DNA sequence from the autoradiogram.<br \/>\n(b) Explain why the sequence read from the autoradiogram is complementary to the<br \/>\noriginal sequence.<\/p>\n<p>15. Study the following enzyme purification table and answer the questions that follow:<\/p>\n<table class=\"mobile\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td style=\"width: 64px;\">Step<\/td>\n<td style=\"width: 243px;\">Procedure<\/td>\n<td style=\"width: 132px;\">Total protein (mg)<\/td>\n<td style=\"width: 107px;\">Activity (units)<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 64px;\">1.<br \/>\n2.<br \/>\n3.<br \/>\n4.<br \/>\n5.<\/td>\n<td style=\"width: 243px;\">Crude extract<br \/>\nSalt fractionation<br \/>\nIon exchange chromatography<br \/>\nMolecular exclusion chromatography<br \/>\nAffinity chromatography<\/td>\n<td style=\"width: 132px;\">15,000<br \/>\n4,000<br \/>\n1,500<br \/>\n688<br \/>\n1-75<\/td>\n<td style=\"width: 107px;\">1,50,000<br \/>\n1,38,000<br \/>\n1,15,500<br \/>\n75,000<br \/>\n52,000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>(a) Which step in the purification is most effective, and why?<br \/>\n(b) Which of the procedures is least effective, and why?<\/p>\n<hr \/>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"SECTION_C\"><\/span><strong>SECTION C<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>16. What is OKT-3? Why is it administered to patients undergoing organ transplantation?<br \/>\nWhat is the relevance of fusing an antibody producing B-cell with myeloma cells in<br \/>\nhybridoma technology?<\/p>\n<p>17. What is \u2018Molecular Pharming\u2019? Suggest any four advantages of expressing transgenic<br \/>\nproteins in milk?<\/p>\n<p>18. Name any three resources available from the NCBI and their uses.<\/p>\n<p>19. What is fed-batch culture and what are its benefits in microbial technology? How is it different from a batch culture?<\/p>\n<p>20. Name the special DNA polymerase used in PCR reactions. What are the three basic<br \/>\nsteps of a PCR cycle? Using a single template molecule, how many DNA molecules are<br \/>\ngenerated after 10 cycles of amplification?<\/p>\n<p>21. What are edible vaccines? Give 3 advantages of developing edible vaccines. Which plant<br \/>\npart(s) will be best suited for expressing antigenic transgene?<\/p>\n<p>22. Why is it difficult to culture animal cells as compared to plant or microbial cells? How is the pH and osmolality of the medium monitored arid maintained in animal cell<br \/>\nculturing?<\/p>\n<p>23. Name any four physical and\/or chemical properties of enzymes which might be useful to change by site-directed mutagenesis. Support your answer by taking an example of an engineered protein\/enzyme.<\/p>\n<p>24. A Chronic Myelogenous Leukaemia (CML) patient has been put on a combination drug therapy for the past 2 months. How can the FISH technique be used to monitor the effect of chemotherapy?<\/p>\n<p>25. What are the microbial culture collection centres? Suggest any two benefits. Name a<br \/>\nmicrobial culture centre from India and its location.<\/p>\n<hr \/>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"SECTION_D\"><\/span><strong> SECTION D<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><strong>26. <\/strong>(a) What is the principle of protein fingerprinting? Illustrate major steps.<\/p>\n<p>(b) Who developed this technique?<\/p>\n<p>(c) What are prions?<\/p>\n<p><strong>27. <\/strong>(a) How will you select bacterial cells carrying a recombinant plasmid?<\/p>\n<p>(b) Explain briefly a technique for visual screening of transformed bacteria.<\/p>\n<p>(c) How can E. coli cells be made competent and who developed this method?<\/p>\n<p style=\"text-align: center;\"><strong>OR<\/strong><\/p>\n<p>(a) Enlist the four major steps in a recombinant DNA experiment.<\/p>\n<p>(b) What is the advantage of having a polylinker in a cloning vector?<\/p>\n<p>(c) Name a cloning vector that can be used to clone large DNA fragments (&gt; 1 MB).<\/p>\n<p>28. (a) Describe vector-mediated and vector-less gene transfer in plants.<\/p>\n<p>(b) Why is Agrobacterium tumefaciens regarded as nature\u2019s genetic engineer?<\/p>\n<p style=\"text-align: center;\"><strong>OR<\/strong><\/p>\n<p>(a) Enlist the six major steps in plant tissue culture.<\/p>\n<p>(b) Name a medium commonly used for culturing plant parts and what factors dictate the choice of media.<\/p>\n<\/div>\n<\/div>\n<div>\n<p style=\"text-align: center;\"><strong>These are questions only. To view and download complete question paper with solution install myCBSEguide App from google play store or login to our\u00a0<a href=\"https:\/\/mycbseguide.com\/dashboard\/\">student dashboard<\/a>.<\/strong><\/p>\n<p style=\"text-align: center;\"><b><strong><a class=\"button\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=in.techchefs.MyCBSEGuide&amp;referrer=utm_source%3Dmycbse_bottom%26utm_medium%3Dtext%26utm_campaign%3Dmycbseads\">Download myCBSEguide App<\/a><\/strong><\/b><\/p>\n<\/div>\n<h2><span class=\"ez-toc-section\" id=\"CBSE_Question_Paper_2007_class_12_Biotechnology\"><\/span>CBSE Question Paper 2007 class 12 Biotechnology<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Download class 12 Biotechnology question paper with solution from best CBSE App the myCBSEguide. CBSE class 12 Biotechnology question paper 2007 in PDF format with solution will help you to understand the latest question paper pattern and marking scheme of the CBSE board examination. You will get to know the difficulty level of the question paper.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Previous_Year_Question_Paper_for_class_12_in_PDF\"><\/span>Previous Year Question Paper for class 12 in PDF<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>CBSE question papers 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011, 2010, 2009, 2008, 2007, 2006, 2005 and so on for all the subjects are available under this download link. Practicing real question paper certainly helps students to get confidence and improve performance in weak areas.<\/p>\n<ul>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-physics\/1251\/cbse-last-year-papers\/3\/\">Physics<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-chemistry\/1267\/cbse-last-year-papers\/3\/\">Chemistry<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-mathematics\/1284\/cbse-last-year-papers\/3\/\">Mathematics<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-biology\/1298\/cbse-last-year-papers\/3\/\">Biology<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-accountancy\/1315\/cbse-last-year-papers\/3\/\">Accountancy<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-business-studies\/1727\/cbse-last-year-papers\/3\/\">Business Studies<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-economics\/1327\/cbse-last-year-papers\/3\/\">Economics<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-history\/1869\/cbse-last-year-papers\/3\/\">History<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-geography\/1863\/cbse-last-year-papers\/3\/\">Geography<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-political-science\/1879\/cbse-last-year-papers\/3\/\">Political Science<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-physical-education\/1877\/cbse-last-year-papers\/3\/\">Physical Education<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-computer-science\/1851\/cbse-last-year-papers\/3\/\">Computer Science<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-informatics-practices\/1873\/cbse-last-year-papers\/3\/\">Informatics Practices<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-english-core\/1855\/cbse-last-year-papers\/3\/\">English Core<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-hindi-core\/1865\/cbse-last-year-papers\/3\/\">Hindi Core<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-hindi-elective\/1867\/cbse-last-year-papers\/3\/\">Hindi Elective<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12\/1250\/\">Other Subjects<\/a><\/li>\n<\/ul>\n<p>To download CBSE Question Paper class 12 Accountancy, Chemistry, Physics, History, Political Science, Economics, Geography, Computer Science, Home Science, Accountancy, Business Studies, and Home Science; do check myCBSEguide app or website. myCBSEguide provides sample papers with solution, test papers for chapter-wise practice, NCERT solutions, NCERT Exemplar solutions, quick revision notes for ready reference, CBSE guess papers and CBSE important question papers. Sample Paper all are made available through\u00a0<a href=\"https:\/\/play.google.com\/store\/apps\/details?id=in.techchefs.MyCBSEGuide&amp;referrer=utm_source%3Dmycbse_bottom%26utm_medium%3Dtext%26utm_campaign%3Dmycbseads\"><strong>the best app for CBSE students<\/strong><\/a>\u00a0and myCBSEguide website.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CBSE Question Paper 2007 class 12 Biotechnology\u00a0conducted by Central Board of Secondary Education, New Delhi in the month of March 2007. CBSE previous year question papers with solution are available in myCBSEguide mobile app and cbse guide website. The Best CBSE App for students and teachers is myCBSEguide which provides complete study material and practice &#8230; <a title=\"CBSE Question Paper 2007 class 12 Biotechnology\" class=\"read-more\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2007-class-12-biotechnology\/\" aria-label=\"More on CBSE Question Paper 2007 class 12 Biotechnology\">Read more<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1346,1014,1447],"tags":[345,1582,1527,1342,1659,1631],"class_list":["post-23369","post","type-post","status-publish","format-standard","hentry","category-cbse","category-cbse-question-papers","category-other-subjects-cbse-class-12","tag-biotechnology","tag-cbse-previous-year-paper","tag-cbse-question-paper","tag-class-12","tag-last-year-paper","tag-ten-year-questions-papers"],"yoast_head":"<!-- This site is optimized with the 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