{"id":23347,"date":"2018-12-10T18:07:49","date_gmt":"2018-12-10T12:37:49","guid":{"rendered":"http:\/\/mycbseguide.com\/blog\/?p=23347"},"modified":"2018-12-17T11:12:24","modified_gmt":"2018-12-17T05:42:24","slug":"cbse-question-paper-2014-class-12-biotechnology","status":"publish","type":"post","link":"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/","title":{"rendered":"CBSE Question Paper 2014 class 12 Biotechnology"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_76 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 eztoc-toggle-hide-by-default' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#Last_Year_Question_Paper_Class_12_Biotechnology_2014\" >Last Year Question Paper Class 12 Biotechnology 2014<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#General_Instructions\" >General Instructions:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#SECTION_A\" >SECTION A<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#SECTION_B\" >SECTION B\u00a0<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#SECTION_C\" >SECTION C<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#OR\" >OR<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#SECTION_D\" >SECTION D<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#OR-2\" >OR<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#OR-3\" >OR<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#CBSE_Question_Paper_2014_class_12_Biotechnology\" >CBSE Question Paper 2014 class 12 Biotechnology<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/#Previous_Year_Question_Paper_for_class_12_in_PDF\" >Previous Year Question Paper for class 12 in PDF<\/a><\/li><\/ul><\/nav><\/div>\n<p><strong>CBSE Question Paper 2014 class 12 Biotechnology<\/strong>\u00a0conducted by Central Board of Secondary Education, New Delhi in the month of March 2014. CBSE previous year question papers with solution are available in myCBSEguide mobile app and cbse guide website. The Best CBSE App for students and teachers is myCBSEguide which provides complete study material and practice papers to cbse schools in India and abroad.<\/p>\n<p style=\"text-align: center;\"><strong>CBSE Question Paper 2014 class 12 Biotechnology<\/strong><\/p>\n<p style=\"text-align: center;\"><strong><a class=\"button\" href=\"https:\/\/mycbseguide.com\/downloads\/cbse-class-12-biotechnology\/1849\/cbse-last-year-papers\/3\/\">Download as PDF<\/a><\/strong><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Last_Year_Question_Paper_Class_12_Biotechnology_2014\"><\/span>Last Year Question Paper Class 12 Biotechnology 2014<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<div>\n<div>\n<h3><span class=\"ez-toc-section\" id=\"General_Instructions\"><\/span><strong>General Instructions:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>All questions are compulsory.<\/li>\n<li>There is no overall choice. However, an internal choice has been provided in one question of three marks and two questions of five marks. You have<\/li>\n<li>to attempt only one of the choices in such questions. Question paper contains four sections \u00be A, B, C and D.<\/li>\n<li>Questions No. 1 to 5 are very short answer questions, carrying 1 mark each.<\/li>\n<li>Questions No. 6 to 15 are short answer questions, carrying 2 marks each.<\/li>\n<li>Questions No. 16 to 25 are also short answer questions, carrying 3 marks each.<\/li>\n<li>Questions No. 26 to 28 are long answer questions, carrying 5 marks each.<\/li>\n<li>Use of calculators is not permitted. However, you may use log tables, if necessary.<\/li>\n<\/ul>\n<hr \/>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"SECTION_A\"><\/span><strong> SECTION A<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>1. If the genes involved in fruit ripening are selectively mutated, what commercial importance can this serve?<\/p>\n<p>2. Is it necessary to use a thermostable form of DNA polymerase in dideoxy DNA sequencing? Why or why not?<\/p>\n<p>3. Environmentalists advocate the disuse of chemical insecticides. Suggest an alternative way to improve crop yields.<\/p>\n<p>4. Why is strain preservation important in microbial cell culture?<\/p>\n<p>5. Two laboratories have developed a procedure for downstream processing of an antibiotic. The major difference lies in the number of steps involved. Which one would you prefer and why?<\/p>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"SECTION_B\"><\/span><strong>SECTION B<\/strong><strong>\u00a0<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>6. Restriction mapping of a linear piece of DNA reveals the following EcoRI<br \/>\nrestriction sites :<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" id=\"Picture 1\" class=\"alignnone\" style=\"height: 79px; width: 364px;\" src=\"http:\/\/media-mycbseguide.s3.amazonaws.com\/images\/cbse\/12\/biotechnology\/lyp\/image001.png\" alt=\"CBSE Question Paper 2014 class 12 Biotechnology\" width=\"364\" height=\"79\" \/><\/p>\n<p>(a) This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis and the gel is stained. What is the size of the fragments obtained?<br \/>\n(b) If a 1000 bp of DNA were inserted between the two restriction sites, what will be the size of fragments when step (a) is repeated?<\/p>\n<p>7. What is the effect of SDS on protein structure? How does it facilitate the determination of molecular mass?<\/p>\n<p>8. Name two database retrieval tools. What is their purpose?<\/p>\n<p>9. In a culture of E. coli, the cell population increases from 2.0 \u00d710<sup>6 <\/sup>cells\/ml<\/p>\n<p>to 16 \u00d7 10<sup>6<\/sup> cells\/ml in 30 minutes. What is the generation time of the given culture?<\/p>\n<p>10. Why is the technique for the production of monoclonal antibodies called hybridoma technology? Give an example of a therapeutic monoclonal antibody for breast cancer patients.<\/p>\n<p>11. If Sanger\u2019s dideoxy method shows that the template strand sequence is:<br \/>\n5\u2019 \u2013 TGCAATGCC \u2013 3\u2019 sketch the gel pattern that would lead to this conclusion.<\/p>\n<p>12. You have been provided with a mixture of proteins (A, B, C, and D) in a buffer of pH 6.5. Protein A has a pI of 5.0 and all other proteins have a pI &gt; 7.0. Based on this information, how will you separate protein A from the mixture? Indicate the principle involved also.<\/p>\n<p>13. A scientist determines the complete genome and proteome of a liver cell and a muscle cell from the same person. Would you expect bigger differences in the genome or proteome of these two cell types? Explain.<\/p>\n<p>14. A genetic engineer wants to isolate a gene from a scorpion that encodes a deadly toxin. The ultimate goal is to transfer this gene to a bacterium for commercial production of the toxin. Should he create a genomic library or a cDNA library? Explain<\/p>\n<p>15. A technician in a tissue culture laboratory accidently removed the identification tag of a petri dish containing cells from a cancer biopsy. How can you identify this petri dish among other petri dishes containing normal cells?<\/p>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"SECTION_C\"><\/span><strong>SECTION C<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>16. Why do organophosphates act as selective inhibitors of serine proteases?<\/p>\n<p>Why is the serine hydroxyl at the active site of serine proteases acidic as compared to other serine residues in the enzyme?<\/p>\n<p>17. What are the essential features of a cloning vector? What is the role of<br \/>\n\u2018cos sites\u2019 in phage lambda?<\/p>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"OR\"><\/span><strong>OR<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>What is the principle of blue-white selection for screening transformed E. coli cells?<\/p>\n<p>18. What are DNA chips (or microarray) and how are they used in functional genomics?<\/p>\n<p>19. List three differences between a batch culture and a continuous culture.<\/p>\n<p>20. What are the potential risks and benefits of GM crops? Enlist three for each.<\/p>\n<p>21. Indicate three applications of stem cell technology<\/p>\n<p>22. What is molecular pharming? As compared to the production of desired proteins in bacteria, why might it be advantageous?<\/p>\n<p>23. (a) Why is it useful to search a database to identify sequences that are homologous to a newly determined sequence?<\/p>\n<p>(b) The X-chromosomes in female mammalian cells are homologous to each other. Do you agree?<\/p>\n<p>24. What is metagenomics? Why is this approach gaining importance?<\/p>\n<p>25. Why are animal cells grown in CO<sup>2<\/sup> incubators and not in regular incubators? Do we require similar incubators for culturing microbial cells?<\/p>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"SECTION_D\"><\/span><strong>SECTION D<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>26. (a) Why is sickle cell anemia called a molecular disease? What is the<br \/>\ndefect?<br \/>\n(b) Who developed the technique of protein fingerprinting?<br \/>\n(c) Indicate two ways by which sickle cell anemia can be diagnosed.<\/p>\n<p>27. (a) Enlist the various steps of plant tissue culture.<br \/>\n(b) Name a medium commonly used for culturing plant parts<br \/>\n(explants). Which factors dictate the choice of media?<\/p>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"OR-2\"><\/span><strong>OR<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>(a) Describe vector-mediated and vector-less gene transfer in plants.<br \/>\n(b) Why is Agrobacterium tumefaciens regarded as nature\u2019s genetic engineer?<\/p>\n<p>28. (a) Schematically illustrate the technique of site directed mutagenesis.<br \/>\n(b) What physical and chemical properties of naturally occurring<br \/>\nenzymes might be useful to change by site directed mutagenesis?<br \/>\nGive an example.<\/p>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"OR-3\"><\/span><strong>OR<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>(a) What are the three basic steps of a PCR cycle and at what<br \/>\ntemperatures are they performed?<br \/>\n(b) How can we selectively amplify a DNA fragment?<br \/>\n(c) Give the sequence of two primers (5 nucleotides long) required to amplify the following DNA sequence by PCR:<br \/>\n5&#8242; ATGCCTAGGATCATGC 3\u2019<\/p>\n<\/div>\n<\/div>\n<div>\n<p style=\"text-align: center;\"><strong>These are questions only. To view and download complete question paper with solution install myCBSEguide App from google play store or login to our\u00a0<a href=\"https:\/\/mycbseguide.com\/dashboard\/\">student dashboard<\/a>.<\/strong><\/p>\n<p style=\"text-align: center;\"><b><strong><a class=\"button\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=in.techchefs.MyCBSEGuide&amp;referrer=utm_source%3Dmycbse_bottom%26utm_medium%3Dtext%26utm_campaign%3Dmycbseads\">Download myCBSEguide App<\/a><\/strong><\/b><\/p>\n<\/div>\n<h2><span class=\"ez-toc-section\" id=\"CBSE_Question_Paper_2014_class_12_Biotechnology\"><\/span>CBSE Question Paper 2014 class 12 Biotechnology<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Download class 12 Biotechnology question paper with solution from best CBSE App the myCBSEguide. CBSE class 12 Biotechnology question paper 2017 in PDF format with solution will help you to understand the latest question paper pattern and marking scheme of the CBSE board examination. You will get to know the difficulty level of the question paper.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Previous_Year_Question_Paper_for_class_12_in_PDF\"><\/span>Previous Year Question Paper for class 12 in PDF<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>CBSE question papers 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011, 2010, 2009, 2008, 2007, 2006, 2005 and so on for all the subjects are available under this download link. Practicing real question paper certainly helps students to get confidence and improve performance in weak areas.<\/p>\n<ul>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-physics\/1251\/cbse-last-year-papers\/3\/\">Physics<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-chemistry\/1267\/cbse-last-year-papers\/3\/\">Chemistry<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-mathematics\/1284\/cbse-last-year-papers\/3\/\">Mathematics<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-biology\/1298\/cbse-last-year-papers\/3\/\">Biology<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-accountancy\/1315\/cbse-last-year-papers\/3\/\">Accountancy<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-business-studies\/1727\/cbse-last-year-papers\/3\/\">Business Studies<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-economics\/1327\/cbse-last-year-papers\/3\/\">Economics<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-history\/1869\/cbse-last-year-papers\/3\/\">History<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-geography\/1863\/cbse-last-year-papers\/3\/\">Geography<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-political-science\/1879\/cbse-last-year-papers\/3\/\">Political Science<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-physical-education\/1877\/cbse-last-year-papers\/3\/\">Physical Education<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-computer-science\/1851\/cbse-last-year-papers\/3\/\">Computer Science<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-informatics-practices\/1873\/cbse-last-year-papers\/3\/\">Informatics Practices<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-english-core\/1855\/cbse-last-year-papers\/3\/\">English Core<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-hindi-core\/1865\/cbse-last-year-papers\/3\/\">Hindi Core<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12-hindi-elective\/1867\/cbse-last-year-papers\/3\/\">Hindi Elective<\/a><\/li>\n<li><a href=\"http:\/\/mycbseguide.com\/downloads\/cbse-class-12\/1250\/\">Other Subjects<\/a><\/li>\n<\/ul>\n<p>To download CBSE Question Paper class 12 Accountancy, Chemistry, Physics, History, Political Science, Economics, Geography, Computer Science, Home Science, Accountancy, Business Studies, and Home Science; do check myCBSEguide app or website. myCBSEguide provides sample papers with solution, test papers for chapter-wise practice, NCERT solutions, NCERT Exemplar solutions, quick revision notes for ready reference, CBSE guess papers and CBSE important question papers. Sample Paper all are made available through\u00a0<a href=\"https:\/\/play.google.com\/store\/apps\/details?id=in.techchefs.MyCBSEGuide&amp;referrer=utm_source%3Dmycbse_bottom%26utm_medium%3Dtext%26utm_campaign%3Dmycbseads\"><strong>the best app for CBSE students<\/strong><\/a>\u00a0and myCBSEguide website.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CBSE Question Paper 2014 class 12 Biotechnology\u00a0conducted by Central Board of Secondary Education, New Delhi in the month of March 2014. CBSE previous year question papers with solution are available in myCBSEguide mobile app and cbse guide website. The Best CBSE App for students and teachers is myCBSEguide which provides complete study material and practice &#8230; <a title=\"CBSE Question Paper 2014 class 12 Biotechnology\" class=\"read-more\" href=\"https:\/\/mycbseguide.com\/blog\/cbse-question-paper-2014-class-12-biotechnology\/\" aria-label=\"More on CBSE Question Paper 2014 class 12 Biotechnology\">Read more<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1346,1014,1447],"tags":[345,1582,1527,1342,80,1631],"class_list":["post-23347","post","type-post","status-publish","format-standard","hentry","category-cbse","category-cbse-question-papers","category-other-subjects-cbse-class-12","tag-biotechnology","tag-cbse-previous-year-paper","tag-cbse-question-paper","tag-class-12","tag-last-year-papers","tag-ten-year-questions-papers"],"yoast_head":"<!-- This site is optimized with the 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