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CBSE Class 12 Biotechnology Syllabus 2022-23

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CBSE Class 12 Biotechnology Syllabus 2022-23 includes Recombinant DNA technology, Protein Structure and Engineering, Genomics, Proteomics and Bioinformatics, Microbial Cell Culture and its Applications, Plant Cell Culture and Applications, Animal Cell Culture and Applications etc for the session 2022 – 2023. Here is the detailed syllabus. To download class 12 Biotechnology CBSE latest sample question papers for the 2023 exams, please install the myCBSEguide App which is the best mobile app for CBSE students. The myCBSEguide app not only provides you the CBSE class 12 Biotechnology model question papers but it also provides class 12 Biotechnology chapter-wise test papers, class 12 Biotechnology best revision notes and other study material for class 12 Biotechnology students.

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CBSE Class – 12
Bio-Technology (Code No. 045)
Syllabus (2022-23)

One Paper
Max Marks:
Time: 3 Hours

Unit VProtein and Gene Manipulation40
Unit VICell Culture and Genetic Manipulation30

One paper
Time: 3 hrs.
Total Marks: 70

Unit-V Protein and Gene Manipulation [40 Marks]

Chapter-1: Recombinant DNA Technology
Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of
recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction
(PCR), DNA Sequencing.
Chapter-2: Protein Structure and Engineering
Introduction to the world of proteins, Structure-function Relationship in proteins,
Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)
Chapter-3: Genomics, Proteomics and Bioinformatics
Gene prediction and counting, Genome similarity, SNPs and Comparative genomics,
Functional genomics, Proteomics, Information sources, Analysis using bioinformatics

Unit-VI Cell Culture and Genetic Manipulation [30 Marks]

Chapter-1: Microbial Cell Culture and its Applications
Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of
microbial growth, Isolation of microbial products, Strain isolation and improvement,
Applications of microbial culture technology.
Chapter -2: Plant Cell Culture and Applications
Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture,
Transgenic plants with beneficial traits, Biosafety of transgenic plants.
Chapter-3: Animal Cell Culture and Applications
Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell


Note: Every student will be required to do the following experiments during the
academic session.

  1. Use of special equipment in biotechnology experiments
  2. Isolation of bacterial plasmid DNA
  3. Detection of DNA by gel electrophoresis
  4. Estimation of DNA by UV spectroscopy
  5. Isolation of bacteria from curd & staining of bacteria
  6. Cell viability assay using Evan’s blue dye exclusion method
  7. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it.
  8. Reading of a DNA sequencing gel to arrive at the sequence
  9. Project work

Scheme of Evaluation

Time: 3 Hours
Max. Marks 30
The scheme of evaluation at the end of the session will be as under:

ATwo experiments6+6 (only one computer based practical)
Practical record04
Viva on Practical04
BProject work
Write up05
Viva on project05

Note:- More emphasis should be given on hands on work in projects.

Prescribed Books:

  1. A Text Book of Biotechnology– Class XII : Published by CBSE, New Delhi
  2. A Laboratory Manual of Biotechnology- Class XII : Published by CBSE, New Delhi
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